transgenic sheep production

The transgenic-positive rate did not significantly differ between the two groups (Table 1; Figs. New polymorphism in the 5' flanking region of. no more than one email per day or week based on your preferences. of recipients transferred; survival rate: no. All transgenic sheep production and sample collection procedures strictly followed the protocols approved by the Animal Welfare Committee of China Agricultural University (Permission Number: SYXK [Beijing] 2015002; the period of valid days: September 22, 2015–September 22, 2020), and the study was conducted in strict accordance with the guidelines and regulations established by this committee. Naunyn Schmiedebergs Arch Pharmacol. All transgenic-positive lambs were identified by sequencing. Rexroad, C. E. and Powell, A. M. (1988) Co-culture of ovine ova with oviductal cells in medium 199. Murray, J. D., Nancarrow, C. D., and Ward, K. A. Minghui Yang conceived and designed the experiments, performed the experiments, contributed reagents/materials/analysis tools, approved the final draft. The endoscope (Karl Storz Endoskope GmbH, Tuttlingen, Germany) intrauterine horn method was used to inseminate donor ewes with 0.2–0.5 mL of fresh semen from five different rams (motility > 0.6) at the 54th h after CIDR removal. Walker, S. F., Seamark, R. F., Quinn, P., Warnes, G. M., Ashman, R. J., Smith, D. H., and Ancell, P. (1988) Culture of pronuclear embryos of sheep in a simple medium. Superovulation efficiency of the transgenic offspring. Embryo transfer efficiencies of frozen/non-frozen microinjected pronuclear embryos and the positive rate of the offspring. The open pulled straw (OPS) vitrification method has been successfully applied in mouse, pig, and goat embryos as well as in buffalo oocytes, but it has not yet been applied to the microinjected embryos. I've been betting with these guys for more than three years and in that time I've made nearly £500,000! In mammals, melatonin secreted by the pineal gland is important for regulating seasonal reproduction (Robinson et al., 1992a). Not logged in Springer Nature is developing a new tool to find and evaluate Protocols. The experimental ewes were White Suffolk, aged 1–4 years, without reproductive diseases and fed on a selected experimental farm for at least 1 month to achieve a healthy condition (55–90 kg). Pregnancy rate, lambing rate, survival rate, average birth weight and transgenic positive rate as well as reproduction efficiency and hormone level of the transgenic offspring were investigated to analyze the effect of OPS vitrification on microinjectd pronuclear embryos. Artificial insemination was performed using semen freshly collected by an artificial vagina from five rams, and diluted 1:1–4 with sheep semen diluent (glucose 31 g/L, sodium citrate 14 g/L, and neomycin sulfate 1 g/L). PeerJ promises to address all issues as quickly and professionally as possible. Walton, J. R., Murray, J. D., Marshall, J T., and Nancarrow, C. D. (1987) Zygote survival in gene transfer experiments. Transgenics showed a small but significant increase in bulk, but male transgenics had a lower staple strength than female transgenics and non-transgenics which did not differ significantly. After thawing, 122 embryos were viable and the survival rate was 89.1% (122/137). Transgenic efficiency is an important factor in evaluating transgenic technology. Rexroad, C. E., Jr. and Wall, R. J. To our knowledge this is the first reported improvement in a production trait by genetic engineering of a farm animal without adverse effects on health or reproduction. The following information was supplied regarding data availability: The raw data are provided in a Supplemental File. The forward primer AA8-F product for AANAT was located in the insertion sequence (forward: 5′-GTCCAGCACTTCCTGACCCT-3′, backward: 5′-CATCAGAAGTTAAA CAGCACAGTTAG-3′). Rexroad, C. E. and Powell, A. M. (1988) Co-culture of ovine eggs with oviductal cells and trophoblastic vesicles. These animals should eventually prove to be valuable sources of proteins for human therapy. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. The serum was separated from the blood and stored at −80 °C prior to hormone analysis. In addition, whether freezing affects the transgenic efficiency or offspring development remains unclear. The work load is far greater and the rates of success far less by most criteria. NIH Guoshi Liu conceived and designed the experiments, performed the experiments, authored or reviewed drafts of the paper, approved the final draft. The effects of a specific transgenic gene on vitrified-thawed microinjected pronuclear embryos have not been reported in any animals. These differences were species-specific as the litter size order is mice > sheep > humans. Many investigators have successfully produced transgenic animals by pronuclear microinjection; however, the efficiency was considerably low. Importantly, the transgenic offspring that overexpressed the AANAT gene showed improved ovulation efficiency and lambing rate by regulating their hormone levels. These keywords were added by machine and not by the authors. Epub 2014 May 16. First, approximately five embryos were pretreated in equilibration solution (ES) solution (10% ethylene glycol (EG) and 10% dimethyl sulfoxide (DMSO) in PBS) for 30 s, then transferred into the EDFS30 solution (15% EG, 15% DMSO, 18% (w/v) Ficoll (MW, 70,000) and 0.3 M sucrose in PBS). The melatonin assay method was performed as described by Zhao et al. This process is experimental and the keywords may be updated as the learning algorithm improves. Five transgenic lambs resulted from the microinjection of 591 embryos; one male and one female showed IGF1 expression in the skin. 2015 May;388(5):583-6. doi: 10.1007/s00210-014-1075-0. Crozet, N. (1991) Manipulation of oocytes and in-vitro fertilization. For example, AANAT transgenic tomatoes and rice exhibited significantly higher melatonin levels than did their wild-types (Okazaki et al., 2009; Park et al., 2013; Kang et al., 2010; Wang et al., 2014). of offspring; No. (1988) Techniques for the transfer of foreign genes into animals. Epub 2014 Dec 2. Superovulation in the donors was induced by injecting 320 IU of PMSG (Ningbo Hormone Products CO., Ltd, Ningbo, Zhengjiang, China) 12 h after sponge removal. For the recipients, their CIDRs were removed 10 h in advance of the donor’s CIDR withdrawal, and the recipients were injected with a single dose of 280–320 IU PMSG. The pregnancy and lamb survival rates of the frozen embryos were lower than those of the non-frozen embryos, while the average birth weight was reversed between the two groups (Table 1).

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